Still trying to assemble ingredients for my media. Thank you for your patience!

Although I do have my tools lined up:

Containers will be half-pint jelly jars, because they're the right size and can take autoclaving.

My sterile cabinet will be a clear plastic rubbermaid-type tote with hand-holes cut in it.

I've got access to a nice analytical balance accurate to 0.0001 gram yay.

Sterilizing agents will be bleach spray and a spirit lamp for flaming off tools.

The autoclave is a beefy old pressure cooker that has a HUGE capacity, but it's at work and I'm still hunting around salvation army locations for a cheap canning processor.

I've narrowed my plant-parts choices to leaf segments and nodes.
We all know that nodes are the source of good totipotent cells when it comes to cloning.
But I want to try leaves as well because obviously the plant has plenty of leaves and not so many 'extra' nodes. Leaf sections will be 1cm square and placed cuticle-side down on the medium so the stomata can still breathe.
stinkyattic Reviewed by stinkyattic on . Stinky's Adventures in Micropropagation... Hey all, I just went out and finally got a digicam with flash so I can document my latest folly. I've decided to let my plants be guinea pigs for a go at tissue culture, which I'm just going to call TC from now in because I type really not so fast! :D Here's what I am trying to find out in these experiments: 1) What media/additive combination works best for shoot formation in Cannabis. 2) What media/additive combination works best for rooting. Rating: 5