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09-07-2006, 01:40 PM #4OPSenior Member
Stinky's Adventures in Micropropagation...
Still trying to assemble ingredients for my media. Thank you for your patience!
Although I do have my tools lined up:
Containers will be half-pint jelly jars, because they're the right size and can take autoclaving.
My sterile cabinet will be a clear plastic rubbermaid-type tote with hand-holes cut in it.
I've got access to a nice analytical balance accurate to 0.0001 gramyay.
Sterilizing agents will be bleach spray and a spirit lamp for flaming off tools.
The autoclave is a beefy old pressure cooker that has a HUGE capacity, but it's at work and I'm still hunting around salvation army locations for a cheap canning processor.
I've narrowed my plant-parts choices to leaf segments and nodes.
We all know that nodes are the source of good totipotent cells when it comes to cloning.
But I want to try leaves as well because obviously the plant has plenty of leaves and not so many 'extra' nodes. Leaf sections will be 1cm square and placed cuticle-side down on the medium so the stomata can still breathe.
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