Hey all,
I just went out and finally got a digicam with flash so I can document my latest folly.

I've decided to let my plants be guinea pigs for a go at tissue culture, which I'm just going to call TC from now in because I type really not so fast!

Here's what I am trying to find out in these experiments:

1) What media/additive combination works best for shoot formation in Cannabis.

2) What media/additive combination works best for rooting.

3) What part(s) of a Cannabis plant are appropriate for TC:
-Nodes
-Internodal stem segments
-Growing tip tissue
-Leaves
-Roots (I might not use roots, depends how much space I have in the incubator)

4) What sort of growth pattern do you get- Can you get a single-stemmed plant out of a mass of TC'd organisms without damaging them too much?

5) How long does this take?

I'm working on a trial media and have come up with a list of components that several different sources that all agree on:
-A gelling agent
-A sugar
-A nutrient source
-One or more growth regulating substances:
-----inositol
-----B1 Thiamin
-----NAA
-----coconut milk (seriously)
-----Kinetin
-----IBA

So I'm putting this all together.

I found Agar locally at a Chinese market as an ingredient in a dessert mix... but it was almond-flavored and already had the sugar added, so no control over that.
GNC stores carry powdered inositol and Thiamin.
Coconut milk comes from coconuts.
NAA and IBA are found in rooting agents but I want them individuially and might have to go to a biological supply store for that.
I don't know where in the heck I'm going to easily find kinetin!

Keep ya posted.
stinkyattic Reviewed by stinkyattic on . Stinky's Adventures in Micropropagation... Hey all, I just went out and finally got a digicam with flash so I can document my latest folly. I've decided to let my plants be guinea pigs for a go at tissue culture, which I'm just going to call TC from now in because I type really not so fast! :D Here's what I am trying to find out in these experiments: 1) What media/additive combination works best for shoot formation in Cannabis. 2) What media/additive combination works best for rooting. Rating: 5