Tissue Culture- Let's discuss!

[stinkyattic]

I want to get a tissue culture discussion going here!
It's my newest little geek obsession...

Here's what I've got so far:

Good points:
You can get thousands of exact genetic replicas from one donor plant with minimum stress to the donor.
You don't need to use a slip with multiple nodes!
You could overnight someone an envelope with a few leaves and it's like getting a clone, you just have to do the rest of the work. So spreading your favorite genetics to your buds (hee hee) would be VERY easy.

Bad points:
Takes 8 weeks
Requires you to be a wicked nerd with a degree in geekiness and thick glasses and no social life (Um, I made that one up)
Requires sterile conditions
Requires media and chemicals that aren't all that easy to find

My questions:
Have any of you happy gardeners done tissue culture on any plant at all?
If so, have you done it on cannabis?
If so, is there a specific supplement that you add to your agar?

Please peeps don't tell me this is a bogus subject since taking cuttings is so easy. Yeah, yeah, yeah, I know, whatever. I'm not arguing that. I mean, f'real, if you want low tech, plant a seed outdoors. Wait for it to rain! This whole hobby is high tech and I think tissue culture is, frankly, COOL.

stinkyattic Reviewed by stinkyattic on 08-30-2006. Tissue Culture- Let's discuss! I want to get a tissue culture discussion going here! It's my newest little geek obsession... Here's what I've got so far: Good points: You can get thousands of exact genetic replicas from one donor plant with minimum stress to the donor. You don't need to use a slip with multiple nodes! You could overnight someone an envelope with a few leaves and it's like getting a clone, you just have to do the rest of the work. So spreading your favorite genetics to your buds (hee hee) would be Rating: 5

[UnitedParcelSecrets]

Oh I agree! Unfortunately, I'm nowhere near geek enough to actually be able to do it, but I think it's awesome. Have YOU done it? And if you have, how'd it turn out? Was it worth the work?

You could be the resident DNA guru (kind of already are).

[stinkyattic]

I haven't done it and I'm trying to assemble enough rudimentary knowledge to try.

[stinkyattic]

Here's something interesting!
DYI media recipe included!

http://www.une.edu.au/agronomy/AgSSrHortTCinfo.html

[UnitedParcelSecrets]

It seems to me, that by doing this ... you could theoretically make a plant that would have many many main colas, or something similar. Couldn't you?

You absolutely have to try this, Stinky!!! I'm so curious.

[stinkyattic]

I 'm assembling all the media, etc as we speak. Some is kind of hard to get your hands on, and I have to wait for my boss to get back from vacation and use his lab account to order some of the stranger chemicals!
One thing I found by doing a literature review is that there is a lot of research being done on micropropagation of hops because it is such a disease-prone genus. Hops, well we all know that's one of the family Cannabacea so I assume the methods are cross-applicable. I'm definitely going to have to kind of wing it on media based on info for hops and info that I found about pot growth regulators.

[Neojedi]

This is quite an interesting subject.From my experience with shrooms, contamination was the biggest problem.

[maik]

Why even use things from "work". This can only bring questions. If not from your boss, maybe his bosses boss.....all it takes is one accountant seeing a strange order/expenditure, and unless you've started a business with "cloned" houseplants, only bad things can be assumed.

Actually, there are plenty of companies out there selling home culture kits, and with the "magic" stuff like growth promoters and such that you'll need.

I've done a bunch of tissue culture, and even protoplast fusion, but that was in University.

But I've never tried just sitting there with a paper puch, holing up some nice, squat indicas to get a bunch of sweet "clones".

And tissue culture IS the same as conventional cloning....same thingy. What will you do when you have lots of callous, though...?

[stinkyattic]

The original plan was just to keep dividing the callous and putting bits of it in a root-promoting media as needed, and keeping the rest of it essentially in suspended animation on shoot-promoting media so it is sort of a genetic archive. I think it's likely I will break down and order a kit... in the meantime I got an ez-clone and am having fun with that.

[harris7]

What types of cells are you going to try starting from. Root, stem leaf? are you going to try all of em for fun?

is cannabis different than other plants in the sence that you can clone from any tissue?