by the way, I would use an electroporator for the gene delivery.....no chemicals and doesn't kill the cells like ...also MUCH cheaper than a BioRad gene gun!!!! Think you can't even buy them, have to lease it with a service contract....and since I have several of their "high tech" molecular bio equipment, like a real time PCR....have to say, it's not worth the hassle...electroporators work great and can be found cheaply on LabX....although I already have one...and a good one at that....