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02-09-2007, 10:08 PM #31Junior Member
Tissue Culture- Let's discuss!
Stinkyattic, It's very good meet you. One thing that I used in the past that is very important is using H2O2 (Hydrogen Peroxide) in the agar. Agar turns solid at (if I remember ) 110 deg - 130 deg. Over 120 deg H2O2 turns into water and hydrogen. The H2O2 must be added to the agar at around 120 deg. H2O2 stops anything from germinating (a great help). In mushrooms you can use this when cloning but not when germinating spores. The way you do this is to leave your boilling flask of agar in the pressure cooker. Remove the lid every now and then to take the temp of the water inside with a thermometer (keeping pressure cooker inside the air hood or if it's to big keep it in the air flow). When temp hits 120 deg pour the H2O2 into the flask, add enough H2O2 to make 10% of total volume. Move flask into hood and put cooker away. Pour agar into petri dishes, put covers on and let cool. Then take a new box of small zip lock baggies. Spray it down with rubbing alcohol and put it into the hood. Put cooled petri diskes into baggies. Spray down a new box of large zip lock baggies and put smaller bagges inside. Store away in frig until needed. Ware rubber gloves and respray hands with rubbing alcohol before putting them into the hood every time. (Don't Smoke) I have alot of agar on hand but it's the wrong mix for plants. I'll have to do some reading. Later, Stash
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