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06-02-2006, 05:24 PM #1Senior Member
Physiological differences in Sativa/Indica stains
Originally Posted by THE MEDIC
Yes, sort of, although CB1 receptors are found throughout the body (this 2002-released study says "expressed primarily in the brain and in some peripheral tissues" (Howlett et al., 2002; Pertwee and Ross, 2002)). CB2 "mainly" in the immune system.
This study does show a difference between the two receptors by administering a CB1 receptor antagonist and it did not affect the effects of the Cannabidiol:
The addition of CBD to the culture medium led to a dramatic drop of mitochondrial oxidative metabolism [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide test] and viability in glioma cells, in a concentration-dependent manner that was already evident 24 h after CBD exposure, with an apparent IC50 of 25 µM. The antiproliferative effect of CBD was partially prevented by the CB2 receptor antagonist N-[(1S)-endo-1,3,3-trimethylbicyclo[2,2,1]heptan-2-yl]-5-(4-chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide (SR144528; SR2) and {alpha}-tocopherol. By contrast, the CB1 cannabinoid receptor antagonist N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboximide hydrochloride (SR141716; SR1), capsazepine (vanilloid receptor antagonist), the inhibitors of ceramide generation, or pertussis toxin did not counteract CBD effects. We also show, for the first time, that the antiproliferative effect of CBD was correlated to induction of apoptosis, as determined by cytofluorimetric analysis and single-strand DNA staining, which was not reverted by cannabinoid antagonists.
First published on November 14, 2003beachguy in thongs Reviewed by beachguy in thongs on . Physiological differences in Sativa/Indica stains Are there any? I've noticed that I only get red-eye when I smoke Sativa strains. Indica strains have no effect on my eyes. The same applies to my speech, coordination, etc. Anyone else noticed this? Or could it be a biological thing? Rating: 5
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