I can't thank you's all enough for your help. I have the rooms sealed up, and now fingers crossed. I just stepped into the veg room to check the temp. While there, I took a leaf from one of my ladies and examined it closely. I can't see any bugs or eggs on this leaf, I was thinking that was good, maybe the veg ladies didn't have them too bad. But then I saw the roads on the underside of the leaf. Are they caused by spider-mites! It does not look good.