Yes it was nuked. After nuking and crotching, the temp would drop down to ~96. From experience I prefer to walk into the collection site with the bottle right at 100F to account for temp drop into the cup.

What I meant was that after the initial nuke it took a little bit of time to come up to the temp which I like to see it at (100F) from the 94-96 range using only the heating pad and body heat.. I think part of the problem was that I didn't have my testing underwear as tight against my body as I should have and I was a bit rusty.

Did another drop at concentra this week (for testing at Alere) using clean human substitute that when straight from their body into my spare QF bottle. Temp drop for the urine into the bottle took it down to 96 and I threw it in the microwave for 5sec to bring it up just above 100F. It held 100F all the way to the collection facility.

Hope that clarifies my response a bit.