Quote Originally Posted by IgotQuestions
My bad, it always seems like they write the line higher when I've taken DOT tests as opposed to non split tests.
They are not allowed to draw a random line on the collection cup either. That would be in violation of Appendix A to Part 40 of the DOT regs. Under Appendix A, the collection cup must have graduated markings already on the cup clearly noting 45 ml and above.

Appendix A to Part 40 - DOT Standards for Urine Collection Kits
The Collection Kit Contents

1. Collection Container

a. Single-use container, made of plastic, large enough to easily catch and hold at least 55 mL of urine voided from the body.

b. Must have graduated volume markings clearly noting levels of 45 mL and above.
..And for the "split" bottles...

Plastic Specimen Bottles

a. Each bottle must be large enough to hold at least 35 mL; or alternatively, they may be two distinct sizes of specimen bottles provided that the bottle designed to hold the primary specimen holds at least 35 mL of urine and the bottle designed to hold the split specimen holds at least 20 mL.

b. Must have screw-on or snap-on caps that prevent seepage of the urine from the bottles during shipment.

c. Must have markings clearly indicating the appropriate levels (30 mL for the primary specimen and 15 mL for the split) of urine that must be poured into the bottles

Source: DOT Rule 49 CFR Part 40 Appendix A | Department of Transportation
The actual regulation covering the required volume of sample:

Subpart E - Urine Specimen Collections
§ 40.65 What does the collector check for when the employee presents a specimen?

As a collector, you must check the following when the employee gives the collection container to you:

(a) Sufficiency of specimen. You must check to ensure that the specimen contains at least 45 mL of urine.

(1) If it does not, you must follow “shy bladder” procedures (see §40.193(b)).

(2) When you follow “shy bladder” procedures, you must discard the original specimen, unless another problem (i.e., temperature out of range, signs of tampering) also exists.

(3) You are never permitted to combine urine collected from separate voids to create a specimen.

(4) You must discard any excess urine.

Source: DOT Rule 49 CFR Part 40 Section 40.65 | Department of Transportation
Burnt Toast Reviewed by Burnt Toast on . Question about preemployment Hello I have to take a preemployment test at Quest labs within a few weeks (as soon as tomorrow) and I definitely would not pass (smoked yesterday, wasn't really sure when i'd be called in). Its for a summer job I've been at for a few years now but now I'm going back earlier. Usually I would just stop for 3 weeks and I'd be clean. Now I either want to dilute or substitute. It's a mandated drug test through the coast guard, which is what makes me question using substitution. It's a split sample Rating: 5