I had found the info youve posted above when i had google searched days ago but i wasnt sure how up to date the info was for 2012 so that was why i started the thread.
All of the regs outlined are the current regs, as of this writing.


Also about the 45ml is good enough, the testing facility i was at was very busy with 5 small cubical booths for collectors with 5 small bathroom doors accross from each booth where i could see other donors going in and out of there bathroom doors and one donors cup i saw had a sharpie mark up almost 2 inch which was where the temp strip was on his cup(roughly 3-3.5 oz) my guess,, these were pretty big cups probably to prevent spillage. But my cup and one other were at about the same spot where the temp strips were and the sharpie marks which was around 1 inch up on the cup..
If what youre saying is true, then you did not take a DOT test. Under the regulations, the collection cup must have graduated markings clearly noting 45 ml and above. The collector cannot just draw a random line on a collection cup and say "fill to here".
Appendix A to Part 40 - DOT Standards for Urine Collection Kits
The Collection Kit Contents

1. Collection Container

a. Single-use container, made of plastic, large enough to easily catch and hold at least 55 mL of urine voided from the body.

b. Must have graduated volume markings clearly noting levels of 45 mL and above.

c. Must have a temperature strip providing graduated temperature readings 32–38 °C/90–100 °F, that is affixed or can be affixed at a proper level on the outside of the collection container. Other methodologies (e.g., temperature device built into the wall of the container) are acceptable provided the temperature measurement is accurate and such that there is no potential for contamination of the specimen.

d. Must be individually wrapped in a sealed plastic bag or shrink wrapping; or must have a peelable, sealed lid or other easily visible tamper-evident system.

e. May be made available separately at collection sites to address shy bladder situations when several voids may be required to complete the testing process.
With DOT testing, regulations and protocols on site, collection, process, analysis and interpreting of the results have to be completely adhered to. Failing to do so would result in serious sanctions imposed by the Agency, including the loss of certifications to perform DOT testing. Since DOT testing is primarily a labs bread and butter, this would spell the end for them as a business.