Quote Originally Posted by Burnt Toast
How do you know this for sure?

Better run a second test on it - but this time use a dropper. Not only do you need to worry about reagent bleed-off into the QF, but you need to worry about bleed-off cross-contamination of the neighboring panels when the strip is withdrawn vertically from the small opening of the QF bottle.
Even the labs use a dropper to avoid a cross-contamination condition.

When using a dropper, drop thick beads of sample on each panel pad and be sure to tip the strip on its side (on a paper towel) to drain off excess.
well i guess i dont know for sure but i cant see anything with my eyes. lol. and it was literally in the quick for as short of time as possible. but ty for advice once agin burnt