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12-24-2009, 02:50 AM #6Junior Member
Hello, I'm Puppydawg...
Okay, I'll start with what Carol Stiff of Kitchen Culture Kits has to say about Cannabis Tissue Culture:
On Thu, Apr 24, 2008 at 4:33 AM, Carol Stiff <kck@...> wrote:
> I have a legal request for medical marijuana culture. I usually tell
> growers to treat the plant like tomato or potato and culture nodes in MS
> medium with no hormones. Any other suggestions. This person is using it
> for severe arthritis pain.
>
> Thanks.............carol
>
> Carol M. Stiff, Ph.D.
>
> <[email protected]?subject=>
So I did some further research and found some tomato protocols
And an excellent report here:
Based on this information, I'm going to try a medium of MS, 1mg/l BAP for callus inducement. For regeneration: N6 medium, no hormones. For plantlet elongation: MS media, no hormone. For rooting: 1/2 strength MS, no hormone. Two teaspoons of sugar in medium except root inducement decrease dosage to 1.5 Tbs.
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