Hi Greenstoner,

Hope all is going well with the patent.

I have been reviewing your references myself and there's a few gaps in my understanding that I wanted to clarify with you, if possible.

1. Geranyl pyrophosphate: geranyl transferase. Fellermeier 98 seems to mostly be a Michaelis-Menten style kinetics study. I cannot find any molecular genetic information on this enzyme, or any nucleotide sequences of it. Do you have a nucleotide sequence for this enzyme? If not, how do you accomplish the olivetolic acid + GPP -> CBGA prenylation biosynthetically?

2. Olivetolic acid synthase. Raharjo, 2004 attempted to assemble the enzyme based off a homologous nucleotide sequence. But they failed to do so, and instead the enzyme exhibited chalcone synthase activity and did not yield olivetolic acid. What enzyme are you using to yield the olivetolic acid? Is the nucleotide sequence for it available?

Thanks for reading. Best of luck on your patent!