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03-04-2010, 05:01 AM #1
Senior Member
DOT P test
Your references are outdated - made long before the current methodologies mandated by DHHS/SAMHSA/DOT:Nitrites can be readily detected by a urinalysis dipstick test or through direct chemical analysis. Because nitrites do not interfere with immunoassays, samples containing concentrations of THC above the threshold will be routinely assayed for the THC-COOH metabolite by GC/MS. A urine sample that exhibits little or no recovery of a deuterated IS or target drug is highly suggestive of an adulterant such as nitrite (2).
Using elemental analysis, mass spectrometry (for pyridine), ion chromatography (for chloride), and inductively coupled plasma atomic emission spectroscopy, the active ingredient in Urine Luck was determined to be PCC. Urine samples adulterated with PCC tested by GC/MS will produce results that are similar to those for nitrite, i.e., low or no recovery of the IS or target drug. This adulterant differs from nitrite, however, because at high concentrations, the chemical also produces negative immunoassay screening results. Thus, drug testing laboratories unaware of the presence of PCC will not perform GC/MS analysis, and the use of PCC may escape detection. Its presence can be suspected by the presence of an abnormally low pH or the appearance of an orange tint to the urine.
So basically if you can get through without a GC/MS run you may be ok. If not, this stuff will destroy the internal standard which sets off a red flag that the sample may contain an adulterant. An inexperienced GC/MS operator could misinterpret results like this but I have a feeling someone more experienced would have to sign off on it and thus, would recognize.
References:
(1)Alan H.B. Wu,a, Ben Bristol, Karen Sexton, Gina Cassella-McLane, Verena Holtman and Dennis W. Hill. "Adulteration of Urine by "Urine Luck" Clinical Chemistry 45: 1051-1057, 1999;
(2)ElSohly MA, Feng S, Kopycki WJ, Murphy TP, Jones AB, Davis A, Carr D. A procedure to overcome interferences caused by the adulterant "Klear" in the GC-MS analysis of 11-nor-9-THC-9COOH. J Anal Toxicol 1997;21:240-242
Which brings us to....Subpart F - Drug Testing Laboratories
§ 40.95 What are the adulterant cutoff concentrations for initial and confirmation tests?
(a) As a laboratory, you must use the cutoff concentrations for the initial and confirmation adulterant testing as required by the HHS Mandatory Guidelines and you must use two separate aliquots â?? one for the initial test and another for the confirmation test.
(b) As a laboratory, you must report results at or above the cutoffs (or for pH, at or above or below the values, as appropriate) as adulterated and provide the numerical value that supports the adulterated result.
[65 FR 79526, December 19, 2000, as amended at 73 FR 35970, June 25, 2008]
With all this pointed out, my original point still stands. Additive use is not recommended if youre taking a DOT test. They will be detected.Adulterated
The following criteria have been established to report a specimen as adulterated:
(1) The pH is less than 3 or greater than or equal to 11 using either a pH meter or a colorimetric pH test for the initial test on the first aliquot and a pH meter for the confirmatory test on the second aliquot;
(2) The nitrite concentration is greater than or equal to 500 mcg/mL using either a nitrite colorimetric test or a general oxidant colorimetric test for the initial test on the first aliquot and a different confirmatory test (e.g., multi-wavelength spectrophotometry, ion chromatography, capillary electrophoresis) on the second aliquot;
(3) The presence of chromium (VI) is verified using either a general oxidant colorimetric test (with a greater than or equal to 50 mcg/mL chromium (VI)-equivalent cutoff) or a chromium (VI) colorimetric test (chromium (VI) concentration greater than or equal to 50 mcg/mL) for the initial test on the first aliquot and a different confirmatory test (e.g., multi-wavelength spectrophotometry, ion chromatography, atomic absorption spectrophotometry, capillary electrophoresis, inductively coupled plasma-mass
spectrometry) with the chromium (VI) concentration greater than or equal to the LOD of the confirmatory test on the second aliquot;
(4) The presence of halogen (e.g., bleach, iodine, fluoride) is verified using either a general oxidant colorimetric test (with a greater than or equal to 200 mcg/mL nitrite equivalentcutoff or a greater than or equal to 50 mcg/mL chromium (VI)-equivalent cutoff) or halogen colorimetric test (halogen concentration greater than or equal to the LOD) for the initial test on the first aliquot and a different confirmatory test (e.g., multiwavelength
spectrophotometry, ion chromatography, inductively coupled plasma-mass
spectrometry) with a specific halogen concentration greater than or equal to the LOD of the confirmatory test on the second aliquot;
(5) The presence of glutaraldehyde is verified using either an aldehyde test (aldehyde present) or the characteristic immunoassay response on one or more drug immunoassay tests for the initial test on the first aliquot and GC/MS for the confirmatory test with the glutaraldehyde concentration greater than or equal to the LOD of the analysis on the second aliquot;
(6) The presence of pyridine (pyridinium chlorochromate) is verified using either a general oxidant colorimetric test (with a greater than or equal to 200 mcg/mL nitrite equivalent cutoff or a greater than or equal to 50 mcg/mL chromium (VI)-equivalent cutoff) or a chromium (VI) colorimetric test (chromium (VI) concentration greater than or equal to 50 mcg/mL) for the initial test on the first aliquot, and GC/MS for the confirmatory test with the pyridine concentration greater than or equal to the LOD of the
analysis on the second aliquot .
Sources:
http://www.dot.gov/ost/dapc/NEW_DOCS...5_20080625.pdf
http://www.workplace.samhsa.gov/Drug...ary%202005.pdf
Drug TestingBurnt Toast Reviewed by Burnt Toast on . DOT P test I gotta take a DOT p test in 10 days. I'm reading all these threads & still confused on what to get & or do. I'm 5'8" 215lbs & a heavy user, been for years. I see QF 4.2 is what most are doing(which is now 5.7) how many of you out there faild this QF? I also see this omnie stuff, sounds like this might not be for me. Anyways, any replys would be great. Rating: 5To view the Board Rules: http://boards.cannabis.com/introduce...ard-rules.html
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