DOT P test

[Burnt Toast]

If the DOT test is a followup/return to duty test due to a previous DOT test being positive for a drug, you will be observed for the followup test (see § 40.67 of the DOT regulations).

And keep in mind that for the observed test, they will check for artificial prosthetic delivery systems and synthetic urine/substitution containers. Under § 40.67, the donor must lower trousers, undergarments, roll the shirt up, and showing the observer by turning around 360 dg, that he/she is not carrying such devices. If no devices are found, then the observer and donor proceeds to enter the bathroom stall. The observer is to directly watch the flow of urine exit the body and into the sample cup.

Before the followup/return to duty test, the donor must complete the SAP process as outlined under Subpart O of 49CFR Part 40 of the regulations.

[Burnt Toast]

i passed a supervised d.o.t. p test by using the urinator.

If you were able to use the Urinator system, you did not take a DOT test. As stated, observers are required by the regs to check for such devices. Read post #5 in this thread:
http://boards.cannabis.com/drug-test...test-soon.html

[rockinrobin]

it was dot all they did was go in the restroom with me it was pre employment . it was 4 yrs ago it might be different now all i was doing was saying what happened to me but dont tell me what it wasn,t you were not there. what your talking about sounds like a fatel accident observed or newer rules.

[Burnt Toast]

it was dot all they did was go in the restroom with me it was pre employment . it was 4 yrs ago it might be different now all i was doing was saying what happened to me but dont tell me what it wasn,t you were not there. what your talking about sounds like a fatel accident observed or newer rules.

If its 4 years ago that you used the Urinator, then thats a different story for that the new D/O rules werent implemented until 2008. You wouldve noticed this if you took time out to follow the links that Ive provided.
With the new rules in place, its very irresponsible to even suggest using the Urinator.

[rockinrobin]

i could of followed the links and you could of asked me how long ago i took the test instead of saying i didn't take it. you got negative first all i did was say what happened to me. you put out a lot of good info. take care

[Burnt Toast]

i could of followed the links and you could of asked me how long ago i took the test instead of saying i didn't take it.

No, thats hogwash! Nobody should have to ask you a damn thing. .... You shouldve included in your original post the first time that this happened 4 years ago. Instead, your post gave off all the vibes that this test took place recently.

[toolenit]

Hello again everyone. I'm still looking for information on the product Spike by Clear Choice. I can't use fake pee because of the way my employer conducts the random drug testing policy. A supervisor will drive us to the collection site so there is no time to mix powdered pee or bring a substitute up to body temp. Could anyone give me information on Spike and when it was used? A sales rep. at Clear Choice says it may have an odor to it. Anyone know? Foley Services is the collecting agent. Does anyone have any info on them? It's a new policy for my employer so no one has any information on how the testing is done. A split specimen is taken. We are not sure if they send both samples to a lab then do a EMIT test or if the EMIT is done right after collection. Sorry I ask for so much but I am new to this. ANY information that would work in my situation would be greatly appreciated. I am subject to a DOT test. Thanks

[smokez4u2]

Drop-in additives like the Urineluck are detected on a more frequent basis than ever before. They are detected when the sample is flagged for high chromates, excessive nitrites, and/or an abnormal pH.

Youd be better off bringing in some premixed synthetic urine, or a clean persons sample. If youre brave enough to sneak in an additive, then youre brave enough to sneak in a sub sample.

Nitrites can be readily detected by a urinalysis dipstick test or through direct chemical analysis. Because nitrites do not interfere with immunoassays, samples containing concentrations of THC above the threshold will be routinely assayed for the THC-COOH metabolite by GC/MS. A urine sample that exhibits little or no recovery of a deuterated IS or target drug is highly suggestive of an adulterant such as nitrite (2).

Using elemental analysis, mass spectrometry (for pyridine), ion chromatography (for chloride), and inductively coupled plasma atomic emission spectroscopy, the active ingredient in Urine Luck was determined to be PCC. Urine samples adulterated with PCC tested by GC/MS will produce results that are similar to those for nitrite, i.e., low or no recovery of the IS or target drug. This adulterant differs from nitrite, however, because at high concentrations, the chemical also produces negative immunoassay screening results. Thus, drug testing laboratories unaware of the presence of PCC will not perform GC/MS analysis, and the use of PCC may escape detection. Its presence can be suspected by the presence of an abnormally low pH or the appearance of an orange tint to the urine.

So basically if you can get through without a GC/MS run you may be ok. If not, this stuff will destroy the internal standard which sets off a red flag that the sample may contain an adulterant. An inexperienced GC/MS operator could misinterpret results like this but I have a feeling someone more experienced would have to sign off on it and thus, would recognize.

Nothing says friendship like borrowed urine

References:
(1)Alan H.B. Wu,a, Ben Bristol, Karen Sexton, Gina Cassella-McLane, Verena Holtman and Dennis W. Hill. "Adulteration of Urine by "Urine Luck" Clinical Chemistry 45: 1051-1057, 1999;

(2)ElSohly MA, Feng S, Kopycki WJ, Murphy TP, Jones AB, Davis A, Carr D. A procedure to overcome interferences caused by the adulterant "Klear" in the GC-MS analysis of 11-nor-9-THC-9COOH. J Anal Toxicol 1997;21:240-242

[Burnt Toast]

Nitrites can be readily detected by a urinalysis dipstick test or through direct chemical analysis. Because nitrites do not interfere with immunoassays, samples containing concentrations of THC above the threshold will be routinely assayed for the THC-COOH metabolite by GC/MS. A urine sample that exhibits little or no recovery of a deuterated IS or target drug is highly suggestive of an adulterant such as nitrite (2).

Using elemental analysis, mass spectrometry (for pyridine), ion chromatography (for chloride), and inductively coupled plasma atomic emission spectroscopy, the active ingredient in Urine Luck was determined to be PCC. Urine samples adulterated with PCC tested by GC/MS will produce results that are similar to those for nitrite, i.e., low or no recovery of the IS or target drug. This adulterant differs from nitrite, however, because at high concentrations, the chemical also produces negative immunoassay screening results. Thus, drug testing laboratories unaware of the presence of PCC will not perform GC/MS analysis, and the use of PCC may escape detection. Its presence can be suspected by the presence of an abnormally low pH or the appearance of an orange tint to the urine.

So basically if you can get through without a GC/MS run you may be ok. If not, this stuff will destroy the internal standard which sets off a red flag that the sample may contain an adulterant. An inexperienced GC/MS operator could misinterpret results like this but I have a feeling someone more experienced would have to sign off on it and thus, would recognize.
References:
(1)Alan H.B. Wu,a, Ben Bristol, Karen Sexton, Gina Cassella-McLane, Verena Holtman and Dennis W. Hill. "Adulteration of Urine by "Urine Luck" Clinical Chemistry 45: 1051-1057, 1999;

(2)ElSohly MA, Feng S, Kopycki WJ, Murphy TP, Jones AB, Davis A, Carr D. A procedure to overcome interferences caused by the adulterant "Klear" in the GC-MS analysis of 11-nor-9-THC-9COOH. J Anal Toxicol 1997;21:240-242

Your references are outdated - made long before the current methodologies mandated by DHHS/SAMHSA/DOT:

Subpart F - Drug Testing Laboratories
§ 40.95 What are the adulterant cutoff concentrations for initial and confirmation tests?

(a) As a laboratory, you must use the cutoff concentrations for the initial and confirmation adulterant testing as required by the HHS Mandatory Guidelines and you must use two separate aliquots â?? one for the initial test and another for the confirmation test.
(b) As a laboratory, you must report results at or above the cutoffs (or for pH, at or above or below the values, as appropriate) as adulterated and provide the numerical value that supports the adulterated result.
[65 FR 79526, December 19, 2000, as amended at 73 FR 35970, June 25, 2008]

Which brings us to....

Adulterated
The following criteria have been established to report a specimen as adulterated:
(1) The pH is less than 3 or greater than or equal to 11 using either a pH meter or a colorimetric pH test for the initial test on the first aliquot and a pH meter for the confirmatory test on the second aliquot;

(2) The nitrite concentration is greater than or equal to 500 mcg/mL using either a nitrite colorimetric test or a general oxidant colorimetric test for the initial test on the first aliquot and a different confirmatory test (e.g., multi-wavelength spectrophotometry, ion chromatography, capillary electrophoresis) on the second aliquot;

(3) The presence of chromium (VI) is verified using either a general oxidant colorimetric test (with a greater than or equal to 50 mcg/mL chromium (VI)-equivalent cutoff) or a chromium (VI) colorimetric test (chromium (VI) concentration greater than or equal to 50 mcg/mL) for the initial test on the first aliquot and a different confirmatory test (e.g., multi-wavelength spectrophotometry, ion chromatography, atomic absorption spectrophotometry, capillary electrophoresis, inductively coupled plasma-mass
spectrometry) with the chromium (VI) concentration greater than or equal to the LOD of the confirmatory test on the second aliquot;

(4) The presence of halogen (e.g., bleach, iodine, fluoride) is verified using either a general oxidant colorimetric test (with a greater than or equal to 200 mcg/mL nitrite equivalentcutoff or a greater than or equal to 50 mcg/mL chromium (VI)-equivalent cutoff) or halogen colorimetric test (halogen concentration greater than or equal to the LOD) for the initial test on the first aliquot and a different confirmatory test (e.g., multiwavelength
spectrophotometry, ion chromatography, inductively coupled plasma-mass
spectrometry) with a specific halogen concentration greater than or equal to the LOD of the confirmatory test on the second aliquot;

(5) The presence of glutaraldehyde is verified using either an aldehyde test (aldehyde present) or the characteristic immunoassay response on one or more drug immunoassay tests for the initial test on the first aliquot and GC/MS for the confirmatory test with the glutaraldehyde concentration greater than or equal to the LOD of the analysis on the second aliquot;

(6) The presence of pyridine (pyridinium chlorochromate) is verified using either a general oxidant colorimetric test (with a greater than or equal to 200 mcg/mL nitrite equivalent cutoff or a greater than or equal to 50 mcg/mL chromium (VI)-equivalent cutoff) or a chromium (VI) colorimetric test (chromium (VI) concentration greater than or equal to 50 mcg/mL) for the initial test on the first aliquot, and GC/MS for the confirmatory test with the pyridine concentration greater than or equal to the LOD of the
analysis on the second aliquot .




Sources:
http://www.dot.gov/ost/dapc/NEW_DOCS...5_20080625.pdf
http://www.workplace.samhsa.gov/Drug...ary%202005.pdf
Drug Testing

With all this pointed out, my original point still stands. Additive use is not recommended if youre taking a DOT test. They will be detected.

[toolenit]

Hello, I'm back to work now and still curious about the new drug testing policy. I spoke with someone that had to submit a urine sample>it is a DOT compliance drug test< and he said that they put dye in the sample after he passed it to them. Does anyone know what the dye is for?