Most home THC tests utilize a 50 ng/ml cutoff level, which is the same cutoff level as the initial immunoassay screen on DOT tests.

With DOT tests, a 5 panel immunoassay screen is done first. If a urine sample tests negative on all 5 drug panels of the screen, the test is over as a "pass", no GC/MS performed. The GC/MS is only done to confirm any positives showing on the screen.

If your home test is negative using your first void of the morning, then you'll be good to go for the DOT test.