I run my system at 60 sec on and 4 minutes off, it all depends on what kind of misters you have and what pressure they are at. If they deliver alot of water and not much air then you could get away with longer times between watering, mine delivers at 100 psi and atomizes inside the grow chamber to make only a very fine mist, contains tons off oxygen. but they dry out faster and need more frequent watering time, i have tried to bump them up to 10 mins and they were wilting like crazy.