Tissue Culture

[oldmac]

lol, don't worry. Chia pets are surprisingly difficult- after all, it IS a hydroponic medium akin to hydtroton... I wonder if they pre-soak the critters at the factory and set them at 6.0 before sale?

Stinkyattic,

Glad you have a sence of humor, guess it helps if your a mod.
I am new here and have spent more time reading then posting, and have figured out in all that reading that you are one smart person. I don't think I have seen you give an answer or advice that I could disagree with. Sticky kudos to ya. Oh, and belated thanks in helping a old man get his thread where it belonged.

In my reading I cam across a Jan 2, 08 post of yours indicating you where going to buy a tissue culture kit. Also seems you expressed interest in this before. So......
did you get a kit?
progress with it?

[stinkyattic]

You know what, I never ended up getting one. I'm still interested, but gearing up for the spring vegetable season kinda got me sidetracked. I need to get past this crazy busy summer season first, lol! Also, I switched to rapidrooter plugs for my cloning and had such good success that I didn't feel the need to learn another complicated procedure quite yet. Probably in fall when the farmin' quiets down and I have some time to devote to learning a new method.
Thanks for the kind words, too.

[oldmac]

Well I ordered one, so we'll get to see if it can be done.
I promise to keep you posted.

I know on one hand it's complicated, but seems if it can be made to work it would save; space, time and tedious work. I get less and less pleasure out of doing cuttings and it don't help with my aching fingers.

The big plus it will force me to learn something new,
probably have to read a few books on the subject too.

[Weedhound]

Yep, that looks like something I could certainly zip right through as well.

[jon6942]

texas grass,

Thanks for the above info, but as you can see they where dealing with cauliflower.
The technique itself I am familiar with; I have seen and helped with african violets and it is truly amazing. While it requires a sterile enviorment, I can do a small laminar flow hood myself (my groom room is almost sterile anyway) and I have a electric pressure cooker to serve as an atuoclave.

But I don't want to do research here.....I want to apply this technology to my grow.
I am willing to experiment tho....
but I need to know out of the many grow mediums, which works best with MJ.
Also, what part of the plant is it best to take cell material.
Which hormones and chems at what stage.
etc, etc.

I need info specific to the plant. Lucky for me hemp is legal for use in many areas and is commerically viable. Currently I'm sifting thru various tech papers and writings from Universties trying to find the info I crave.

I would love to be able to grow REAL CLONES not cuttings, in test tubes, petri dishes and baby food jars on a few feet of counter space in my work area, then having to keep 6 mature and 2-3 up and comming mothers for each strain I want to run. I currently switch between two strains....so I keep 16 or so plants, just for cuttings.

I know there's a better way, just gotta get the info.

ok so i was reading this and found this chinese study about what they used.
http://www.pakbs.org/pjbot/PDFs/41%2...%282%29603.pdf
i hope this is whatur looking for seemd tdz is best but im a big noob @ this just started

[bullyslayer]

thats quiet a wash they gave those seeds

[thrive]

update? have you had time to run experiments?

can you now discuss:
1 jars,
2 media,
3 hormones,
4 chems
5 kits

or maybe a simple cannabis "kitchen culture kit" review?

found this via google... nice topic
Thanks m8 :thumbsup:

p.s. i am looking for the perfect jars and gel for the jars... ive seen things on youtube that dont discuss what the chemical gel is.

also... are there <3 organic <3 alternatives to this?

[oldmac]

Hey there Thrive,

I got the African Violet micro-propagation down but never had any luck working out the formula for mj. Plus was busy this past summer with medical problems.

In fact I did not see the post from jon6942 this past August....just saw it now. I'm going to go over the paper and see if it makes sence.

This is still a fasinating area but I haven't found anyone doing it with mmj.

[getglazed]

yes it is possible - after much reading, building etc etc. I gave it a try

Some photos of success and failure. If contaminated, you will know in a min of 3 days and by 7 days you will see the fog/mold starting.

or you will see growth like in the Jack Herer attached.

I am trying a tissue culture on my best Blueberry plant I have seen in a while. about 20 different seeds but there was one with the best smell, bud definition and the buzz that made you see colors.

of course, i did not get a clone of this one and hope the Tissue culture can save the day

Much Respect

[thrive]

cool pic Glazed!
Can you tell us about the jars your using, and the medium ? etc?
thanks!

also, what plant bodypart are you using?