Two things...

It's almost impossible to regenerate cannabis plants from tissue culture callus. Sucks, but it's true. Then again, I'm not sure how many different strains people have tried, and there's often a lot of genetic variation regarding this.

Secondly... "florigen" has been found to not be a small molecule, but rather an mRNA message that's sent from the leaves to the flower primordia. So grinding up callus and applying them to plants to try to get them to flower won't work. Bummer, eh?

Quote Originally Posted by canoeboat
so what exactly are you trying to modify if you don't mind me askin?

i've always thought it would be cool to get a cell culture going and then differentiate the cells into a specific type via the ratio of hormones. I'd then flower, perhaps by taking a sample of a flowering leaf off of an actual plant and dropping it into the culture, which would transfer the unknown "florigen" compound and jumpstart flowering. Once flowering, well it'd be pretty sweet to only get a certain part of plant cell develope, and you wouldn't waste energy/time on growing things like vascular cells and roots that don't have much use. *now this is all assuming it is practical in a real world setting. perhaps legally?

[on florigen transfer]<it is something that might be applied onto another live plant. I've never tried it before but i bet you could make a leave mass and rub it on an incision on a plant just starting to flower and have a relatively low-stress flower booster, or maybe you could grow it under longer lighting than 12h and increase photosynthesis as long as you still apply the flowering concoction and the right plant hormones and nutrients in some way. >

anyone one have experience grafting a flwoering leaf onto a veging one? I remember reading a study where they did that with a nicotene plant and it worked but i can't find a link right now

!USA=OBAMA!
^hell ya i'm so glad i don't have to move!
Plazmid Reviewed by Plazmid on . Auxin - Indole Acetic Acid Does anyone have any experience with this hormone? I'm looking to conduct an experiment soon in combination with my stomata manipulation I plan on attempting my next grow. I have a load of articles but was curious if anyone has tried using this hormone via root or foliar uptake. I've searched this forum as well as numerous others and I don't come up with any hits except for maybe the occasional definition of auxin is posted and it's listed there briefly. So I'm guessing no one has gone Rating: 5