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02-02-2008, 03:59 PM #15
Senior Member
Florida Biochemist Designs Citrus Tree with THC
if you go to the link through high times to the original page the story's on, it has the steps in how to design one...perhaps if you had more of an understanding of chemistry it may sound more realistic?
Originally Posted by angry nomad
" Biochem 101: How to design a Cannabis-equivalent citrus plant
Step One:
Biochemically isolate all the required enzymes for the production of THC.
Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited. "
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