I'm a CDL holder and I've had 2 DOT physicals and neither doctor ever did the nut grab routine. I don't know for sure, but I would assume if that was part of the DOT requirement they would have done it to me. But like I said, I don't know for sure so I'd feel bad if I said "they don't do that", then they do and find ur QF.

As for the order of how things go...hmm I have a bad memory and I was clean at the time so I didn't even think to remember something like that....but I do think I took the piss test after the physical. The last time I went the doc made me run in place for about a minute to check my blood pressure....that kinda movement might knock loose the QF bottle so I'd make sure you have it extra secure.

I've never had to use QF for a DOT drug test by the way, but I do have a bottle just in case I get one of those randoms....