Anyone store clones in the fridge in a zip lock? If so, what steps do you take?
Adieu
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Anyone store clones in the fridge in a zip lock? If so, what steps do you take?
Adieu
never heard of this bear, but i'd love to know your cloning techniques, if you care to share.
love, brooke
Yea, Bear, how do YOU clone, sir?! How indeedy? :D
:dance:
Oh, and I've never heard of the refridge clone-storing technique...sounds improbable, but not impossible. Let me know how it works out. :thumbsup:
TGF
My cloning tech, hmm. Well, I use CloneX gel, sharp clean snippers and anti wilt spray. My last round I used Rapid ruders, they are an organic clone starter plug that are made out of composted bark and pre soaked to proper PH levels. I wait until the branch I want to take clones from has a couple of inter nodes on the branch and then get close to the tip and snip usually right above an internodes so that once I make the cut that plant wil grow back a few new shoots to take clones from the next round. As soon as I make that cut I place the clone in a dish of water so that no air gets into the cut creating an air bolas that can stop the clone from rooting by stopping nute uptake or water uptake. Then from the water I dip the clone into some antiwilt solution, I don't think that is necessary, but since I have it around I use it, it slows some of the wilting that takes place allot of time after cloning. I then put the clone back into the dish of water and shave some of the skin back where I took the cutting to expose a little more of the root to the medium I will be using to get more uptake, then I dip it into the CloneX and into the rapid ruder. From there I put them in a tray with a dome and a heating pad under the trey, clones seem to take to heat in my exp. Instead of ruders you can use RW cubes or just go right into a soil/perlite mix. I have a 15 sight bubble cloner that I may mess around with this next go round.
Hope this helps some.
On one hand cutting can be a pain in the rear when you firststart , but once you get the hang of it, it is not much trouble. For instance, I was trimming my plants when they were in the first week of flower, and this girl who was bushing up reel nice was one that I didn't take a clone from, and I didn't have all of my cloning things around so I just put some super thrive mixt with water that I keep in a misting bottle and pored it into a cup and snipped a cutting off of the girl and quickly put it root down in the cup. I put it in my computer cab and kind of forgot about it for a week and when I checked on it it was like I had just cut it. I threw it in a soil, perlite mix and moisend the soil a little and it looked like it was going to die a day later, so I pulled it out of the dirt and put a puddle of Super Thrive and water in the hole and misted a zip lock bag and taped it around the little pot and it perked up the next day and is rooted now. I didn't use any clonex, anti wilt spray, and it did just fine.
Adieu
Love you's as well
There is a way to store clones in the fridg, I am just having a hard time catching someone who can shoot me the step by step. Over on IC mag this fellow stored some clones in the fridge for 7 weeks, and others had done it to. Maybe I will just have to experiment a little, or maybe I will just sit some in a glass for a couple weeks and see how they do with changing the water every few days.
My mother cab is getting too crowded and I need to take some clones. I had to give away a Big Bud x Skunk 1 today because I didn't have the room, but at least the guy gave me some Purple Lion clones from Altamet seeds. It was kind of funny, his clones are 2 weeks and they have about 4 leaves and are about 8 inches tall, mine are about 9 inches tall with about 30 leaves and internodes already. My wife thought something was wrong when he left because of the differance, but I told her some folks just do it a little different. I may experiment and cut about 3 inches off of the clones he gave me and reroot them because they are so stretched that it just aint funny, and I want to put them in with some cuttings that I will take next week. Adieu
high!
i have an article here about storing clones in a fridge,,,in fact rooting clones in a fridge!
this is not an easy task, first everything is done micro!, the cuttings are little pieces of stem of leaf, no more than 2 inches long, this is called micro cloning, the next difficult part will be getting hold of the gel used which is calledbasal medium or murashige and skoog [MS],
having presumed you are now aremed with this and some sterile glass tubes you may begin.
firstly, the addvantages,,
storing genetics,
clearing of deseaseand or pests,
fungus and other micro organisms,
and rejuvination of plants,!
the method is very presise, everything has to be very sterile, once the cutting have been taken they too must be sterilised by means of soaking in a solution with 5 ml bleech to 1 litre of water,then washed three times with sterire distilled water,
then they are ready for planting in the glass test tubes in the gel!,once they are tin the tubes, seal the top with alluminium foil, not air tight!,
and place in the fridge,,,,now this is more dificult!,,, the fridge needs to have light!, 18 hrs of course no more than 36 watd cfl or tubes per meter square!, keep the fridge at 12 to 14 degrees centigrade, after 14 days the new micro leaves will apear and after 30 days roots will have formed, once this has hapened the clones can be kept like this forseveral months, as they grow very slowly indeed, when you want to use the clones, remove from the test tube and plant straight into your grow medium,
all of the above process is done in a perfectly sterile laboratory conditions,,
the amount of importance to sterility is paramount!wear surgical gloves! stereilise knive after evry cut! by dipping in alchohol 70% from chemist and burnig off! boil the test tubes in pressure cooker with lid on untill boiling andallow to cool with the lid on!for 24 hrs!, wash everything with alcohol before you start.
the info for this thread was taken from soft secrets issue number two 2006, in spanish and inerperated into english by me!
i think i have covered all the necesarry info, if not try checking out soft
sectrets on line.
it is a free newspaper from holland available threwout europe!
good luck.
welcome to the world of endless possibilities,,,,hydroponics!
mistake the size of the nicro clones is between 3 and five millimeters
1/4 inch
I have herd of that technical way of storing for long term on a micro scale, but I don't have the time or the energy to do all of that, but I will post any new information on my end.
Thanks for the input, and I am going to look up that Soft Secrets for a read on that process.
Adieu
your welcome
OK, found a little info on what I want to try. Figured I would share
Timing clones
by Ed Rosenthal
There are several ways of slowing down the growth of clones. The first is to delay planting. You can store the cuttings in the vegetable chiller section
of a refrigerator in a plastic bag, the way you’d store lettuce for about two weeks or longer. This will weaken the clones a little and they will have
a lower strike ratio. However, it may be a worthwhile trade-off. After the clones are cut they can be left unpruned or trimmed for planting. Then dip them
in a 1% hydrogen peroxide solution to sterilize them to prevent infection. A 1% solution is made by diluting one part drugstore hydrogen peroxide to two
parts water that has been boiled and cooled. When you are ready to plant just take the clones out of the bag.
The cutting state is perilous for plants so a good environment that promotes active growth minimizes risks. Once the cuttings have formed roots and are
growing new leaves, their metabolism can be slowed down. There are a number of ways to do this. Plant metabolism and light requirements slow down in a
cool environment. At 45-50°F (8-10°C) the plants remain in a nearly suspended state. They require only 150 watts of fluorescent per square meter (15 watts
psf) and very dilute fertilizer. When you are ready to jump start them, place them in a warmer environment with brighter light and more fertilizer. They
will quickly respond to the change in the environment.
Even if you can’t cool the clones to 50°F (10°C), you can decrease the intensity of the light and provide diluted water-nutrient mix. The growth rate will
slow.
i do it all the time........just take your cuttings, mist with water,put them in a zip lock freezer bag with a hole in it and put them in the frig. you can keep them like that for about 2 weeks and still have all of them grow. just re cut the stem before you put them in the rapid rooters.......i like to use the rapid rooters too
Yep - I store/ship cuttings like this, but have them completely submerged in water in the baggies, leaf and all. But cutting only, not rooted clones. I have had cuttings viable after 5+weeks using this method, just recut them and use rapid rooters (have you been spying on me Rusty? ;)
I knew that some prophets would come forward with soothing words of truth for me to help me move forward on my journey. Thanks folks
Adieu
a great skill to have, This is my first post here so If y'all will indulge me we could wrap this tread up with a story...
once , way back in the day...the late 60's, actually as I think of it it was more like summer 73 or 74 or so pretty sure 73 because I had a few of the "New" Hps lamps and they just where blowing away our fluros at the time of which we would have banks and banks of ,literally walls of tubes on rollers that could be moved and huge arrays that hung from the ceiling we did some extream LSTing to say the least.
Anyhow one day the home I lived in at the time started to fill with smoke then a fire truck roared up and stopped next door , I thought I was going to get sick, then I saw the fire men running to my neighbors(row home) and started to bash out windows, kinda felt a little relief, then there was banging on our door and panic returned to my stomach I ran the other way and my wife went to the door haha, the police said she had to leave the house she said she was the only one home called the dog and grabbed the cat and locked the door behind her I ran to the basement put bags over 3 plants at a time and cut down everything I had about 20-30 plants and tossed them in my trunk(attached basement Garage thanks gawd) then ran out back ducking by the fence to avoid being seen by Firefighters poking about the house next door and carried about 10x 5 gallon tomato and pepper pots off of the patio 4 at a time bear hug style quickly lined them all up tossed the cut rooted pots in a out of the way closet quick looked for loose leaves and lit a cigarette (yeah i still smoked tobacco back then)hot boxed it to change up the stink blew smoke all over turned off all the lights and split , I met up with my wife at a friends house a few blocks away waiting for a call from the fire chief saying it was safe to return.After a few beers and a joint I had forgotten all about the clandestine mid-day reefer evacuation, but I remembered that I bought a crate of beer the other day and it was still in my trunk , Harold said he would grab it since we needed more ice from the garage also, it took him like half an hour when I finally found him running up and down his back stairway a million times a minute, the beer the bag of ice and all my plants were in the tub filled with cold water, so we ended up drinking beer and taking like 200 clones that night and his wife packed the rest just as she did her dill, or rosemary ect. in a plastic bag with damp napkin they got planted a few days later and most if not all rooted,
before this I had a slight spider mite problem , but I got to bomb the basement and introduce new washed plants a day later.
Of course Harold and I had to meet with the fire chief that afternoon to inspect my house for the damage mostly the roof and a full walk through, when we came to the grow room by that time Harold and I drunk as hell where joking around a lot like why couldn't the fire take my wife and cat and leave me my dog and roof ect. the fire chief wanting a beer of his own no doubt didn't I really was not questioned about the tomatoes, I mumbled something about my wife and heirlooms and state fair contest or some shit he commented only that the set up looked safe and professional enough. I noted my family name ,(my father, owned a Nursery and Garden supply store that at that point he had sold year earlier and had become a small chain , eventually to be bought up by ACE/True value) so the guy left,
Harolds nephew fixed my roof and we set off on rigging more lights for Our growing family,
the moral of this story is that if you break a main stem beyond repair your cat pulls up your favorite plant, no worries toss her in a bag and then into your trunk , drive to a friends , get drunk , put her in the tub and hack the little lady to pieces, then drunkenly deal with the chief when he comes snooping.
Peace and may the Universe Bless you
It's been awhile since I stored clones. But I did place about 40 clones one time in the fridge just to judge how long they'd last. Took 5 clones a week and rooted them. Rooted 5 after 2 weeks - all rooted. The ones taken after 3 weeks - 4 out 5 rooted. After 4 weeks 3 rooted, only one rooted after 5 weeks and I tossed the rest.
It would seem to me that if one were to invest some time trying different solutions in the baggie that this could be improved upon. Lot of work though.
storing clones in fridge is a snap. cut clones as you normally would but keep a lil long if possible. put in ziplock bag and put in crisper drawer in fridge. do NOT mist with water before putting in fridge as this will put you on a road of mold problems which you dont want. take bag out of fridge and do an air exchange in bag atleast once a week. clones can be kept like this for up to 8 weeks. have done this many times and got info off of this site. have not lost a clone as of yet.
I'd like to pass on to everyone the method I use to keep clones (cuttings).
I use a pint size mason jar, and put about 1/2" of water in it. Place cuttings in jar with cut end in water. Pint jar will hold up to abt 60 cuttings.
I vacum seal jar (1/30th atmosphere if your vacum sealer has settings, seems to work best for me) then I place jars in a cardboard box, then into the refrigerator.
I have had great results at 90 days. I have a batch I will check soon when they reach 6 months old and we'll see how it goes when I propagate them.
that sounds great. I plan to travel overseas and I would like to clone my plant and store them until I get back which could be 2 or 3 months. So if I stor them in the fridge like with your method will I need to do anything like water them each week etc? The reason I ask is that I won't have anyone in my house to do this while I'm away so I need a method where I can leave them stored for a few months without any caring.Quote:
Originally Posted by oldmac
This thread is awesome, got a female a grew outside, but have no room to flower, so I'm gonna keep the clones until my current is done flowering. Here's to growing year round!
Is there a book you guys learned this from? Any kind of tutorials you guys can suggest? I'm interested in this.
I cut my clones a couple of nodes longer than usual, seal them into freezer bags, stick them in a plastic lunch box, bag the box and write the date on top, stick it in the crisper draw (7C) for up to 8 weeks. Personally, I haven't gone further than 8 weeks.
When it's time for cloning I let the lunch box acclimatise to room temperature before opening, mist the foliage, recut the stems, and then it's the same process as cutting fresh material.
Takes a few days longer to root and a few days longer to acclimatise to lower humidity, but it's a mighty useful way of making sure you always have clone stock.
Sorry I did not give you an answer sooner.Quote:
Originally Posted by skooozer
You need to do nothing to them, they are vacum sealed. Water has been forced into the cutting from the cut end. Just be carefull not too pull too much vacum or the internal cells may be damaged.
BTW: propagated some at 195 days (6 1/2 months) 95% success rate.
SarahLouise's tip to cutting a little longer first and re-trim the cut is excellent advice too.
:D
I'm going to uy one of those vacuum sealers today and try it out this afternoon! I can't wait! I have 40 m39's that have started the 12/12 today, and are due for a trim on the bottom. (They're under 4 1000w hortiluxes)
They're about 16 inches tall, with anywhere between 6-12 heads each. So, I'm going to cut the lower stems and remove all the little leaves and budding shoots so as to leave about 6-8 stems with 7-8 nodes on each.
I figure that should leave me with about 100 cuttings, more or less. I'd like to keep these for at least two months, and then start to root them for spring. This vacuum sealer idea seems great! I'll come back on and let you guys know how it works for me.
Oldmac, do you have any idea what that would be in degrees? I tried to figure it out but its making my brain hurt too much. I'm going to experiment here pretty soon, and I'll post my results. I'll be using a foodsaver jar attachment and a brake bleeding pump that has a vacuum gauge, cheap, simple, and might just be really effective. I'd like to try 1/30th atmosphere to start with if only I could figure out where thats at on the gauge.
any updates on this thread?
what kind of updates you looking for??
Well anyone get past 6 months preserving clones? When storing in the jar, does it need to be vacuum sealed? and the jars that are vacuum sealed with a bit of water they dont get opened at all for fresh air right?