Tissue Culture

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Originally Posted by oldmac

Has anyone here done successful tissue culture with marijuana, or knows of someone who has.

I am told by experts that mj should be no problem to culture, but I have not been able to find any research or studies into mj tissue culture.

Anybodies help here would be appreciated

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I have a patented cannabis tissue culture media send me an email and lets talk

[email protected]

bill
theberry

We are just getting into it. Here are some plantlets growing from plant stem cells in a media. We haven't raised any full term yet.



Attachment 274101

Graywolf,

Very nice start there. I'm curious how you came up with and how you did work with stem cells. Everything I came across indicated the best cells to use were from the center of the leaf, vein area.

I gave up long ago, just like stinkyattic, I had too much else going on. But my scientific curiosity still finds this process interesting.

Keep up the great work, and please keep us posted as you procede.

OM

Quote:

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Originally Posted by oldmac

Graywolf,

Very nice start there. I'm curious how you came up with and how you did work with stem cells. Everything I came across indicated the best cells to use were from the center of the leaf, vein area.

I gave up long ago, just like stinkyattic, I had too much else going on. But my scientific curiosity still finds this process interesting.

Keep up the great work, and please keep us posted as you procede.

OM

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Our red head is using stem cells from stem nodes.

I will ask him to post a process after he gets it shaken out. Attachment 274143This is his first run, which so far has been successful on a couple of dozen strains, but he hasn't grown any to the point of roots or subsequent adulthood.

Thanks Graywolf,

Since yesterday I kept running thru my head....stem, where would I get cells from? Was thinking maybe cross cut a stem and use pith cells?

This AM I saw your post and eureka.....
NODES, of course, the light bulb went on over my head.

These would be meristem cells or meristematic tissue.

BTW, I had managed to get green growth plantlets started (not as nice as what you showed) but could never round the corner and get roots to grow...the point I finnally gave up at.

Thanks again
OM

You're welcome bro!

Growgeek debates whether it actually even requires the meristem cells, as he has successfully grown plantlets from bottom branch nodes, but has had the best results from the nodes toward the end of the branch.

He plans to move the plantlets to a solution with different hormones and growth regulators to harden them and grow roots. More on that, after we see how it works.