Tissue Culture- Let's discuss!

You can get many of the chemicals and materials you need from Carolina Biological or Fisher Scientific (education section). I would also check techniques used in orchids....the most info on cloning in tissue culture is on these plants. Current methods can allow you to do gene transfers and get plants to produce many medical compounds. I have access to all necessary equipment including ultra low temp storage, sterile hoods, autoclaves....I'm looking into it. Imagine being able to place the gene that produces THC into another plant...what would the Feds do then? Probably prosecute for some new law....or better yet, add a promotor gene to the THC genes, producing massive quantities....:smokin: :thumbsup: I always knew this degree would come in handy for something ....

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Originally Posted by machinehead83

Imagine being able to place the gene that produces THC into another plant...

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Is that even possible? o.O Hot damn, science is amazing.

I wouldn’t smoke it, genes react in unpredictable ways.

Harris... planning on trying all of them to see which works fastest... but hoping for results with leaves especially since there are so many of them...

Machinehead... I've got access to that stuff too, cool. Just trying to figure out if orchid media would be appropriate... I'd love to get the THC gene into hops... it's a vine and a perennial and close already!

Afghooey... yes, I think you have to use bacterial or fungal carriers, but that's a whole field I don't know a damn about!

Harris... I wouldn't smoke it either. Oh well, nice to dream anyway!

i accutaly know quite a bit about GM and yes bacterial fungal viral or the gene gun. which shoots little pieces of gold soaked in DNA into te cells

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Originally Posted by stinkyattic

Harris... planning on trying all of them to see which works fastest... but hoping for results with leaves especially since there are so many of them...

Machinehead... I've got access to that stuff too, cool. Just trying to figure out if orchid media would be appropriate... I'd love to get the THC gene into hops... it's a vine and a perennial and close already!

Afghooey... yes, I think you have to use bacterial or fungal carriers, but that's a whole field I don't know a damn about!

Harris... I wouldn't smoke it either. Oh well, nice to dream anyway!

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Why are there all these people afraid of genes???:dance:

The hops idea is a good one Stinkyattic....I like the idea of inserting a high copy, powerful promotor in to control the gene...I'll bet some pubmed lookups will find the sequence for the gene, even bet there are companies that sell it ready to go....it's REALLY big now to design drugs based on MJ....that is why they will fight legalization with every lawyer and breath in their bodies....I have 1 friend working for a Big Pharma company that has been getting strains from Amsterdam for 7 years and isolating the compounds of different medical strains....finding the best ones for pain and making a drug out of it...etc....

My doc budies are using marinol for MS with GREAT success, but says the patients tell him the real deal works much better....actually stops the autoimmune distruction of mylen sheaths....probably through a very slight inhibition of immune function (remember them trying to blame HIV on MJ? it does lower some T cell function).....OK, I've gotten WAY too geeky....LOL

love to compare notes with you Stinkyattic....:thumbsup:

by the way, I would use an electroporator for the gene delivery.....no chemicals and doesn't kill the cells like ...also MUCH cheaper than a BioRad gene gun!!!! Think you can't even buy them, have to lease it with a service contract....and since I have several of their "high tech" molecular bio equipment, like a real time PCR....have to say, it's not worth the hassle...electroporators work great and can be found cheaply on LabX....although I already have one...and a good one at that....:)

is that were they pass the current through the cells which make them more permeable?


i'm not afraid of genes i'm afraid of uncontrolled genetic Engineering mainly in our food supply. I support it in controlled lab settings to create drugs. As they are controlled. But with all this shit Monsanto is doing they released practically untested, as the US gov doesn’t require it, into the environment.

Yes, an electric pulse is sent through the media the cells are in and carries DNA or RNA into the cell. Also used to get proteins into cells.

As far as plant genetic engineering...you are listening to too much propaganda....there have been NO wierd products produced in this area....they use identified genes for different properties such as frost resistance, sweetness, nutritional value factors....I could see someone with ill intentions using it for some horrible effect, but that is true with anything. You can't prevent every nut out there from doing nasty things. I can also bet that any plant that has been altered required many months if not years in the lab environment just to get it to grow properly. Now if you were talking about insects, animals, humans, etc...I have a REAL problem with messing with that genetic make up. Although it's already being done with farm animals (in labs) in an attempt to make their organs acceptable for transplantation into humans...they put human genes in that express the same HLA antigens as humans so not rejected. These are known genes. You should see all the transgenic animals already available!!! Insects, bacteria, viruses are what you have to worry about, TRUST ME. When you mess with them, that's where the problems begin. messing with plants and farm animals will not put anyone in danger, unless they make a fire breathing mad cow with wings (not possible).

Also, you should know that in the past, regular breeding techniques in insects have created killer bees among other screw ups....we didn't need genetic engineering to do that!

Don't worry, be happy......:rasta:

i dont know what you mean by weird products. I am talking about normal food crops ie canola, corn, soy. Which are all the largest crops in north america and are now GM (not all but lots. all in the case of canola and soy. the only non gm soy is grown in asia)

I can bring up many examples of how they fucked up. Dont tell me i'm listening to propaganda. How about GE corn. which has caused infertility in pigs. And a big warning went out to European farmers telling them not to feed GE corn to pigs they want to breed. Hmm a mammal and what are we... mammals. Yet no warning for us.

It is an incorrect assumption to think that these are being tested properly. They are done by American corporations. And what was Monsanto’s comment to this

Something like: as a corporation we are concerned with selling as much of our product as possible, it is not our responsibility to find out the safety of the products. This is the FDA’s job.

And what did the FDA say. Hmm I believe 15 out of 17 FDA scientists voted againsted releasing these products YET the American government overruled them and released them.

I am willing to speak to this. But I don’t think it is appropriate to discuss in this thread.