Hello, I'm Puppydawg...

[Puppydawg]

I love Cannabis: It has enriched my life in so many ways and enabled me to function even though I have a chronic illness.

I want to change the world with Cannabis: It can relieve peoples' suffering, revive depressed economies, and heal the planet.

I also want very much to micropropigate Cannabis: I'm looking for a feller around here that calls himself "Old Mac"...Anyone seen him?:pimp:

[Dutch Pimp]

welcome ...Dawg....:thumbsup:

http://boards.cannabis.com/advanced-techniques/173946-uvb-experiment.html

[the image reaper]

welcome aboard :thumbsup: ... yup, old Mac is a loved and respected participant here :thumbsup:

[Puppydawg]

Thanks for the welcome! :thumbsup:

Old Mac's name came up a few times while I was doing cannabis tissue culture research; he's actually the reason I joined. I have some information to share with him (and the rest of the class)...

The missing link seemed to be media composition. I think I found it.

[Weezard]

Thanks for the welcome! :thumbsup:

Old Mac's name came up a few times while I was doing cannabis tissue culture research; he's actually the reason I joined. I have some information to share with him (and the rest of the class)...

The missing link seemed to be media composition. I think I found it.

No be shy PD.:)
O. M. is a "name known to all"

You got mo' betta medium dan Coco?
I'm all ears, brah.

D. P. posted da link, so, I''ll see ya ovah dere.
I'm da tiny green one wit da sticky toes.

Aloha
Weezard

[Puppydawg]

Okay, I'll start with what Carol Stiff of Kitchen Culture Kits has to say about Cannabis Tissue Culture:

On Thu, Apr 24, 2008 at 4:33 AM, Carol Stiff <kck@...> wrote:

> I have a legal request for medical marijuana culture. I usually tell
> growers to treat the plant like tomato or potato and culture nodes in MS
> medium with no hormones. Any other suggestions. This person is using it
> for severe arthritis pain.
>
> Thanks.............carol
>
> Carol M. Stiff, Ph.D.
>
> <[email protected]?subject=>


So I did some further research and found some tomato protocols (http://www.planttransformation.ncsu.edu/protocols.asp#tomato)

And an excellent report here: (http://www.baptcb.org/ptc/Full_article/ptc14_1_06.pdf)

Based on this information, I'm going to try a medium of MS, 1mg/l BAP for callus inducement. For regeneration: N6 medium, no hormones. For plantlet elongation: MS media, no hormone. For rooting: 1/2 strength MS, no hormone. Two teaspoons of sugar in medium except root inducement decrease dosage to 1.5 Tbs.

[Puppydawg]

Okay, I'll start with what Carol Stiff of Kitchen Culture Kits has to say about Cannabis Tissue Culture:

On Thu, Apr 24, 2008 at 4:33 AM, Carol Stiff <kck@...> wrote:

> I have a legal request for medical marijuana culture. I usually tell
> growers to treat the plant like tomato or potato and culture nodes in MS
> medium with no hormones. Any other suggestions. This person is using it
> for severe arthritis pain.
>
> Thanks.............carol
>
> Carol M. Stiff, Ph.D.
>
> <[email protected]?subject=>


So I did some further research and found some tomato protocols (http://www.planttransformation.ncsu.edu/protocols.asp#tomato)

And an excellent report here: (http://www.baptcb.org/ptc/Full_article/ptc14_1_06.pdf)

Based on this information, I'm going to try a medium of MS, 1mg/l BAP for callus inducement. For regeneration: N6 medium, no hormones. For plantlet elongation: MS media, no hormone. For rooting: 1/2 strength MS, no hormone. Two teaspoons of sugar in all medium except root inducement decrease dosage to 1.5 Tbs. I have Carols' kit so I measure my agar with a spoon; slightly increase dosage for rooting medium.

I'll be trying this in January and will post results.

[Dutch Pimp]

Hi.. Dawg...post your info in the grow forums (start a new thread, there)...:thumbsup:...(it's wasted here in the INTRO forum..:cool:..)

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