Auxin - Indole Acetic Acid

[daihashi]

Does anyone have any experience with this hormone? I'm looking to conduct an experiment soon in combination with my stomata manipulation I plan on attempting my next grow.

I have a load of articles but was curious if anyone has tried using this hormone via root or foliar uptake.

I've searched this forum as well as numerous others and I don't come up with any hits except for maybe the occasional definition of auxin is posted and it's listed there briefly.

So I'm guessing no one has gone this route, but thought I'd ask for everyone's input just in case.

If anyone is interested I have a crap load of articles on IAA, or I could write an article and my proposed experiment with variables and controls.

Also does anyone here use a spray bottle with a very small micron droplet size. If so let me know where you got it and what the micron size is. Looking for something very small for foliar feed purposes.

[daihashi]

bump on this. Anyone have any experience with it?

[daihashi]

Well that sucks. Seems either no one has experimented with this or researched it well.

I'll be writing an article on this probably this weekend.

I'll include both my stomata and Idole Acetic Acide article in one thread since I'll try to be using both of these to try to manipulate the plants on a cellular level.

Hopefully it goes as I plan.

The actual experiment probably won't occur for another 8-9 weeks (after my harvest is done and i've had time to re-design my grow space.).

[canoeboat]

hello, i am experienced with IIA treatments. Not as much as I would like but I think you and I share similar goals in relation to modifying plants on a cellular level. I have class now so I have to go but I look forward to sharing some ideas with you.

I plan to grow not an authentic plant, but a peatree dish version, contolling all aspects of growth and development through different ratios of plant hormones. I have done this before in a plant cellular and molecular class before but want to adapt it to higher purposes. ok, gotta go peace!

[daihashi]

I plan to grow not an authentic plant, but a peatree dish version, contolling all aspects of growth and development through different ratios of plant hormones. I have done this before in a plant cellular and molecular class before but want to adapt it to higher purposes. ok, gotta go peace!

I'm interested in hearing more about this. What were the results of IAA when introduced and were the effects localized only or were changes seen elsewhere than the point of where uptake occurred?

[canoeboat]

hey sorry i've been away for a bit- it's real busy here not to memtion the election.

i was able to find some of the data posted online for our experiment with giberellin, but i haven't found the info on the IIA yet. I'm in the middle of a last minute assignment right now so i've gotta run but some recent reading has said that willow trees contain significant amounts of plant hormones in their roots, I believe it was more cytokynin not IIA but check into that.

Here is the link to the course I took. It was very general, but the labs kicked ass. The pea plant was hidden traits over generations, but we did more labs than just that. I'm sure you could email dr jensen and ask him any questions, he is a very cool guy.

i'll be back with actual data. peace!

[fadedd]

hey i been wandering about the same thing as u guys...
what u know bout naa...? it is in superthrive and some rooting gels..
i have heard of iaa but need to read up to remember what i know.. smokers lag....lol...... i know that i cut a clone this year from my mother plant and it was really small...i dipped in my rooting horomone and fed with super thrive and the root hormone in da water too..... my test outcome was a thick stemed plant that was very short wid thick branchs.... the leafs also showed signs of its stems bulging more than the leafs on the mother ........ i have been doing the same ever since.... and all outcome are the same for me....

[SOG420]

i am also interested in this subject.Please keep us updated!

[SOG420]

hey sorry i've been away for a bit- it's real busy here not to memtion the election.

i was able to find some of the data posted online for our experiment with giberellin, but i haven't found the info on the IIA yet. I'm in the middle of a last minute assignment right now so i've gotta run but some recent reading has said that willow trees contain significant amounts of plant hormones in their roots, I believe it was more cytokynin not IIA but check into that.

Here is the link to the course I took. It was very general, but the labs kicked ass. The pea plant was hidden traits over generations, but we did more labs than just that. I'm sure you could email dr jensen and ask him any questions, he is a very cool guy.

i'll be back with actual data. peace!

where is this link?

[canoeboat]

sry heres the link to the department

OSU Plant Cellular and Molecular Biology (http://www.biosci.ohio-state.edu/pcmb/)

here's the link to the class i took

OSU Plant Cellular and Molecular Biology (http://www.biosci.ohio-state.edu/pcmb/osu_pcmb/courses_101.php)

i don't think everything's listed on the website but give me a few days and hopefully i can find all my old stuff from the class

[canoeboat]

OSU PCMB101 Laboratory Resources (http://www.biosci.ohio-state.edu/pcmb/osu_pcmb/pcmb_lab_resources/pcmb101_activities/hormones/plnt_grth_hrmns_hormonal_ctrl.htm)

^ there's a nice foto

sorry for the double posting, I've read these forums for some time now, and I've got some log pictures I'll need to be posting shortly. peace!

[WhiskeyTango]

Im followin ya D!!

[canoeboat]

so what exactly are you trying to modify if you don't mind me askin?

i've always thought it would be cool to get a cell culture going and then differentiate the cells into a specific type via the ratio of hormones. I'd then flower, perhaps by taking a sample of a flowering leaf off of an actual plant and dropping it into the culture, which would transfer the unknown "florigen" compound and jumpstart flowering. Once flowering, well it'd be pretty sweet to only get a certain part of plant cell develope, and you wouldn't waste energy/time on growing things like vascular cells and roots that don't have much use. *now this is all assuming it is practical in a real world setting. perhaps legally?

[on florigen transfer]<it is something that might be applied onto another live plant. I've never tried it before but i bet you could make a leave mass and rub it on an incision on a plant just starting to flower and have a relatively low-stress flower booster, or maybe you could grow it under longer lighting than 12h and increase photosynthesis as long as you still apply the flowering concoction and the right plant hormones and nutrients in some way. >

anyone one have experience grafting a flwoering leaf onto a veging one? I remember reading a study where they did that with a nicotene plant and it worked but i can't find a link right now :(

!USA=OBAMA!
^hell ya i'm so glad i don't have to move!

[canoeboat]

here's some good reading

Auxin (http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/A/Auxin.html)

[Plazmid]

Two things...

It's almost impossible to regenerate cannabis plants from tissue culture callus. Sucks, but it's true. Then again, I'm not sure how many different strains people have tried, and there's often a lot of genetic variation regarding this.

Secondly... "florigen" has been found to not be a small molecule, but rather an mRNA message that's sent from the leaves to the flower primordia. So grinding up callus and applying them to plants to try to get them to flower won't work. Bummer, eh?


so what exactly are you trying to modify if you don't mind me askin?

i've always thought it would be cool to get a cell culture going and then differentiate the cells into a specific type via the ratio of hormones. I'd then flower, perhaps by taking a sample of a flowering leaf off of an actual plant and dropping it into the culture, which would transfer the unknown "florigen" compound and jumpstart flowering. Once flowering, well it'd be pretty sweet to only get a certain part of plant cell develope, and you wouldn't waste energy/time on growing things like vascular cells and roots that don't have much use. *now this is all assuming it is practical in a real world setting. perhaps legally?

[on florigen transfer]<it is something that might be applied onto another live plant. I've never tried it before but i bet you could make a leave mass and rub it on an incision on a plant just starting to flower and have a relatively low-stress flower booster, or maybe you could grow it under longer lighting than 12h and increase photosynthesis as long as you still apply the flowering concoction and the right plant hormones and nutrients in some way. >

anyone one have experience grafting a flwoering leaf onto a veging one? I remember reading a study where they did that with a nicotene plant and it worked but i can't find a link right now :(

!USA=OBAMA!
^hell ya i'm so glad i don't have to move!